>formatdb -i ecoli.nt -p F -o T breaks up the ecoli.nt into ecoli.nt.nhr ecolii.nt.nin ecolii.nt.nnd ecolii.nt.nni ecolii.nt.nsd ecolii.nt.nsi ecolii.nt.nsq I do not seems to have blast.txt and formatdb.txt in /opt/BioBrew/NCBI/6.1.0/doc. I guess I'll have to read on on blast when I have the time, but for now I only need to get mpiblast to work. Thank you _________________________________________________________________ Find love on MSN Personals http://personals.msn.com.sg/