Hi, I came across this same error as Khalil, while trying to run any emboss application I get this: error while loading shared libraries: libnucleus.so.0: cannot open shared object file: no such file or directory. The file is located at /opt/BioBrew/EMBOSS/2.6.0/lib/ I followed the instructions: In your ~/.bashrc, write: export LD_LIBRARY_PATH=/opt/BioBrew/EMBOSS/2.6.0/lib:$LD_LIBRARY_PATH then either log out and log back in, or source your ~/.bashrc to export the variable in your shell and the error changed to: EMOSS An error in ajacd.c at line 1534: acdfile '/home/build/BuildRoot-ia32/BUILD/EMBOSS-2.6.0/emboss/acd/pepnet.acd' not opened Is there something else I can do or should I download the other rpm at http://bioinformatics.org/ftp/biobrew/rpms/ Thank you. _________________________________________________________________ Find love on MSN Personals http://personals.msn.com.sg/