Sorry I had not try out the other method mention by David Lapointe -n Base name for BLAST files [String] Optional But mpiBLAST works fine for me if I just edit the DBLIST of (null).nal and save ti as ecoli.nt.nal I will try his method asap. About EMBOSS, I have not tried building from srpm before, so I think I will take some time. Thanks >Hmmm....looks like the package was built with some hard-coded paths. Not >good. I've posted the srpm at http://bioinformatics.org/ftp/biobrew/srpm so >you can try and rebuild it on your cluster frontend yourself. I'll try to >build it myself tomorrow. > >Does this mean you got mpiBLAST to work? > >Glen > >On Jul 22, 2004, at 9:04 PM, kenix y wrote: > >>Hi, >> >>I came across this same error as Khalil, while trying to run any emboss >>application I get this: >>error while loading shared libraries: libnucleus.so.0: cannot open >>shared object file: no such file or directory. >> >>The file is located at /opt/BioBrew/EMBOSS/2.6.0/lib/ >> >>I followed the instructions: >> >>In your >>~/.bashrc, write: >> >>export LD_LIBRARY_PATH=/opt/BioBrew/EMBOSS/2.6.0/lib:$LD_LIBRARY_PATH >> >>then either log out and log back in, or source your ~/.bashrc to export >>the variable in your shell >> >>and the error changed to: >> >>EMOSS An error in ajacd.c at line 1534: >>acdfile >>'/home/build/BuildRoot-ia32/BUILD/EMBOSS-2.6.0/emboss/acd/pepnet.acd' not >>opened >> >>Is there something else I can do or should I download the other rpm at >>http://bioinformatics.org/ftp/biobrew/rpms/ >> >>Thank you. >> _________________________________________________________________ Get 10mb of inbox space with MSN Hotmail Extra Storage http://join.msn.com/?pgmarket=en-sg