>You will need to create one where you run mpiblast, and in your home >directory. Meaning that I need to create .ncbirc in /root, is that right? [NCBI] Data=../data [BLAST] BLASTDB=../db Er, I'm not too sure where the weight matrices are, any way I can find out? >Then edit the BLASTDB=../db line to point to the database (ecoli.nt in your >case). As for ecoli.nt, I've just moved it to /tmp/blast So the line should be [BLAST] BLASTDB=/tmp/blast/ecoli.nt? >You will need a copy of this in you home directory and in the directory you >run the mpiblast code. As for the directory to run my mpiblast code, it can be anywhere? Or is there a specific location? Thanks for all your help _________________________________________________________________ Find it on the web with MSN Search. http://search.msn.com.sg/