I have been running benchmarks with blastall on several different machines. We've come to realize that one of the biggest differences affecting search times is how the machines actually maintain the search databases in memory. Eg.. On our IBM 8-way machine, the databases are held in the memory, which seems to be an effect of the architecture of the machine, and search times become incredibly fast after an initial run, which stores the database in memory. The same effect seems to take place on our Dual Xeon Dell (PE 1650), which also outpaces the Xserves significantly after an initial run to populate the db in memory. It would appear the the Xserves dump the db from memory after each search, even when submitting batch jobs with multiple sequences in a file. Is anyone aware of how this functions, and how this effect might be changed to allow the db to stay in memory longer? Thanks Juan Perin Child. Hospital of Philadelphia