> -----Original Message----- > Subject: [Bioclusters] Seqret > > > HI All, > > I am having an issue with seqret, and was wondering if any of > you have seen it, or know what may be happening. Some requests > work, and others don't. For example, a request using the USA > nt:L32140 returns the sequence, but when I try, for example, > nt:U51243, this is what I get: > > -bash-2.05b$ seqret nt:U51243 > Reads and writes (returns) sequences > Error: Unable to read sequence 'nt:U51243' > Died: seqret terminated: Bad value for '-sequence' and no prompt By any chance is the file which contains U51243 > 2Gb in size? The current version of dbiflat fails to index any sequences past the 2Gb limit in any file and just silently drops them. There is a fix in the works in the next EMBOSS release, but for now the only fix is to split your database files into <2Gb chunks. If this isn't it, try running seqret -debug nt:U51243 and look at the seqret.dbg file for clues as to what's going wrong. Simon.