[BiO BB] Newbie question.[Scanned]

lichunjiang lichunjiang at sibs.ac.cn
Tue Jan 8 01:40:14 EST 2008



Hi, 
yeast one hybrid  will help in your case if you have no
idea which gene will be the target gene.
If you have some idea on
the  target  gene,  Both  EMSA  John mentioned is
a good choice.  

Good Luck!
Lichun

> I
haven't tested the procedure myself, but a paper in 2006 in BMC
>
Bioinformatics described a "machine learning approach." See Yan
et al.
> 7:262. Good luck!
> 
> 	-----Original
Message-----
> 
From: bbb-bounces at bioinformatics.org on
behalf of Donnie Darko
> 	Sent: Sun 1/6/2008 9:57 AM
>
	To: bbb at bioinformatics.org
> 	Cc:
> 	Subject: [BiO BB]
Newbie question.[Scanned]
> 
> 
> 
> 	Hi
folks,
> 
> 	Suppose I have a novel gene whose product is
a DNA binding protein, how
> 	would I go about locating potential
binding sites where this protein
> could
> 	bind? I'm only
interested in finding sites for this specific protein and
> not
> 	a general scan of tfbs.
> 
> 	I've extracted
upstream sequences of genes that I'm interested in but I
> 	can't
think of a way forward.
> 
> 	Cheers and belated happy new
year :-P
> 	_______________________________________________
> 	BBB mailing list
> 	BBB at bioinformatics.org
>
	http://www.bioinformatics.org/mailman/listinfo/bbb
> 
>

> _______________________________________________
> BBB
mailing list
> BBB at bioinformatics.org
>
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> 


-- 
lichunjiang at sibs.ac.cn
Institute of Biochemistry and
Cell Biology,
Shanghai Institutes for Biological Sciences,
Chinese Academy of Sciences



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