How big is ecoli.nt Around 5MB (when I type the command just now, the response is "Trying to break ecoli.nt (5MB) into 3 fragments of 2MB Executing : formatdb -i ecoli.nt -o T -p F -v 2") What happens when you try to format it with regular formatdb? when I /opt/BioBrew/NCBI/6.1.0/bin/formatdb -i ecoli.nt -o T -p F ecoli.nt is broken into fragments and being stored at /home/bigcpuguest but there is no ecoli.nt.nal. _________________________________________________________________ Find gifts, buy online with MSN Shopping. http://shopping.msn.com.sg/